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Transfer of primed CD4 + OX40 − T lymphocytes induces increased immunity to experimental Salmonella typhimurium infections in rats
Author(s) -
THYGESEN PETER,
CHRISTENSEN HENNING BJØRN,
HOUGEN HANS PETTER,
RYGAARD JØRGEN
Publication year - 1997
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1997.tb00588.x
Subject(s) - salmonella , immunity , microbiology and biotechnology , enterobacteriaceae , virology , biology , chemistry , escherichia coli , immunology , immune system , bacteria , genetics , gene
The protective effect of primed CD4 T cells against a lethal dose of Salmonella typhimurium was studied in Lewis rats. Primed CD4 T cells were obtained by inoculating Lewis rats with a non‐lethal dose of S. typhimurium . Four weeks after the infection, spleen non‐adherent mononuclear cells were isolated. The cells were separated according to their expression of CD4 and the OX40 antigen by FACS. OX40 + and OX40 − CD4 + T‐cell subpopulations were together with unsorted CD4 + T cells transferred to untreated rats 24 h prior to infection with S. typhimurium . Transfer of either unsorted CD4 + T cells or CD4 + T cells sorted into OX40 − or OX40 − subpopulations significantly increased animal survival compared to controls. Animals receiving OX40 + CD4 + T cells did not differ significantly in survival probability from those receiving unsorted CD4 + T cells. However, animals receiving OX40 − CD4 + T cells had a significantly better survival compared to animals given unsorted CD4 + T cells. It is concluded that OX40 − CD4 + T cells can induce significant protection against S. typhimurium infections in rats. This is most likely due to the fact that the OX40 − CD4 + T‐cell population contains a significant number of antigen‐specific memory T cells that have returned to a resting state.