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Identification of porcine Pneumocystis carinii as a genetically distinct organism by DNA amplification
Author(s) -
WAKEFIELD A. E.,
KEELY S. P.,
STRINGER J. R.,
CHRISTENSEN C. B. V.,
AHRENS P.,
PETERS S. E.,
BILLEHANSEN V.,
HENRIKSEN S. Aa.,
JORSAL S. E.,
SETTNES O. P.
Publication year - 1997
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1997.tb00576.x
Subject(s) - pneumocystis carinii , biology , gene , polymerase chain reaction , mitochondrial dna , dna , ribosomal rna , nucleic acid sequence , microbiology and biotechnology , sequence analysis , virology , genetics , human immunodeficiency virus (hiv) , pneumocystis jirovecii
DNA was amplified from lung samples from three piglets infected with Pneumocystis carinii , using oligonucleotide primers designed to the P. carinii mitochondrial large subunit ribosomal RNA gene. The nucleotide sequence of the amplification product was determined and indicated lack of sequence variation among these pig‐derived P. carinii samples at this locus. The data showed that porcine P. carinii was genetically distinct from P. carinii isolated from other mammalian host species.