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A xenograft line of human teratocarcinoma established by serial transplantation in severe combined immunodeficient (SCID) mice
Author(s) -
ABE YOSHIYUKI,
OSHIKA YOSHIRO,
OHNISHI YASUYUKI,
SUTO RYUJI,
TOKUNAGA TETSUJI,
YAMAZAKI HITOSHI,
KIJIMA HIROSHI,
HIRAOKA NOBUYOSHI,
UEYAMA YOSHITO,
TAMAOKI NORIKAZU,
NAKAMURA MASATO
Publication year - 1997
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1997.tb00570.x
Subject(s) - teratocarcinoma , pathology , transplantation , immunohistochemistry , biology , stem cell , cellular differentiation , cancer research , medicine , microbiology and biotechnology , biochemistry , gene
We established a xenograft line of human teratocarcinoma (TC‐1) and characterized the pluripotency of differentiation of the neoplastic cells. A teratocarcinoma specimen obtained from a primary mediastinal lesion (22‐year‐old male patient) was inoculated subcutaneously into severe combined immunodeficient (SCID) mice. The carcinoma formed tumors in the mice. We established a xenograft line by serial passage of the tumor in vivo. The primary tumor was composed of papillary and pseudoglandular nests of highly atypical epithelial cells with foci of glomeruloid structures. The metastatic cells showed apparent production of mucin and differentiation to striated muscle. The xenograft line TC‐1 retained the basic histopathological features seen in the primary and metastatic cells. The xenograft line showed focal differentiation to cartilage through serial passages. Immunohistochemical studies with anti‐α‐fetoprotein (AFP) demonstrated positive immunoreactivity on the TC‐1 cells. Serum AFP levels were also elevated in the TC‐1‐bearing SCID mice. The human teratocarcinoma xenograft line TC‐1 will be useful for studying the differentiation mechanism in human totipotent stem cells.