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Production and characterization of mouse monoclonal antibodies against Afipia felis
Author(s) -
ENGBÆK KRÆSTEN,
UTTENTHAL LARS OTTO,
KOCH CLAUS
Publication year - 1997
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1997.tb00558.x
Subject(s) - epitope , antigen , monoclonal antibody , antibody , biology , antiserum , precipitin , felis , microbiology and biotechnology , virology , immunology , cats , medicine
A series of 10 monoclonal antibodies reacting with Afipia felis antigens were selected from mice immunized with live organisms of the reference strain ATCC 53690. Immunoblotting against SDS‐PAGE‐separated A. felis sonicate allowed the antibodies to be classified into three groups: 1) 168‐4, ‐6, ‐7 and ‐10 reacted with a 53 kDa antigen, 2) 168‐1, ‐3 and ‐9 reacted with both 53 kDa and 60 kDa antigens, and 3) 168‐2, ‐5 and ‐9 reacted with other antigens. Antibodies of group 1 did not cross‐react with other Afipia species or 36 unrelated bacteria, whereas those of groups 2 and 3 reacted with other Afipia species and some unrelated bacteria. Immunoblots of crossed immunoelectrophoretic patterns of A. felis sonicate against rabbit antiserum showed that antibodies of groups 1 and 2 bound to the same precipitin arcs. Antibodies of group 1 reacted with a species‐specific epitope on the 53 kDa antigen, while those of group 2 reacted with other epitopes shared by the 53 kDa and 60 kDa antigens. The binding of antibodies of group 1 to A. felis sonicate was inhibited by post‐infection rabbit serum, whereas no inhibition was observed for antibodies of group 2. The species‐specific epitope of the 53 kDa antigen and the early appearance of antibodies against this epitope after infection suggest that this antigen can be used in a serodiagnostic test for A. felis infection.