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Migration of IL‐2‐activated natural killer cells in vitro: influence of extracellular matrix proteins
Author(s) -
Unger M. L.,
Johansson B. R.,
Nannmark U.
Publication year - 1996
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1996.tb04950.x
Subject(s) - chemotaxis , fibronectin , extracellular matrix , matrigel , microbiology and biotechnology , chemistry , laminin , chemokinesis , in vitro , agarose , cell migration , immunology , biology , biochemistry , receptor
An experimental set‐up for estimating a) cellular migration under agarose and b) response to chemoattractant gradients built up in the agarose was used in order to explore the behavior of adherent interleukin‐2 (IL‐2)‐activated natural killer (A‐NK) cells on cell culture plastic and after coating with extracellular matrix (ECM) constituents. A‐NK cells were deposited in wells in the agarose and directed migration, chemotaxis, towards aggregates and suspensions of B16F10 melanoma cells, suspensions of YAC‐1 cells, and tumor‐conditioned media, all deposited in wells at a 2.5 mm distance, was tested. A‐NK cell'chemotaxis was exclusively observed when B16F10 aggregates were used as attractants. The substrate influenced chemotaxis considerably, untreated plastic surface being most favorable for a chemotactic response, followed by laminin, fibronectin, and collagen IV pretreatments. Coating with reconstituted basement membrane matrix (Matrigel®) gave lesser random movements, chemokinesis, of A‐NK cells than coating with the purified components laminin and collagen IV, and the least motile response was obtained after collagen I pretreatment. These in vitro observations indicate that melanoma cell aggregates release humoral factors of a probably short‐lived nature with a chemoattractant effect on A‐NK cells, and that ECM composition influences migratory response, both conclusions with a bearing on the understanding of A‐NK cell infiltration into tumors in vivo.