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Structure, growth and cell proliferation of human osteosarcoma and malignant fibrous histiocytoma xenografts in serial transplantation in nude mice
Author(s) -
BrostrÖM L.Å.,
Crnalic S.,
LÖFvenberg R.,
Stenling R.,
Boquist L.
Publication year - 1996
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1996.tb04942.x
Subject(s) - immunohistochemistry , transplantation , pathology , osteosarcoma , nude mouse , flow cytometry , cell growth , staining , proliferation index , in vivo , bromodeoxyuridine , doubling time , serial passage , biology , cell , cell culture , cancer research , medicine , microbiology and biotechnology , genetics
Tumour specimens from one patient with osteosarcoma and one with malignant fibrous histiocytoma were transplanted in serial passages in nude mice. Structure, growth and cell kinetics of the xenografts were studied in order to assess the validity of the two tumour models. Cell proliferation was analysed using in vivo labelling with the thymidine analogue iododeoxyuridine (IdUrd) and the IdUrd labelling index (LI) was determined by immunohistochemistry. The DNA index (DI) was examined by flow cytometry. The c‐myc oncoprotein expression was studied by immunohistochemistry. More intense proliferation was observed in the peripheral parts of the tumours. There was no correlation between tumour growth and cell proliferation in the two tumour groups. Stability of the tumour models was indicated by low intrapassage and interpassage variations of DI, LI, and volume doubling time, and also by retained histopathological characteristics and c‐myc staining patterns of donor patients' tumours during serial transplantation.