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Increased expression oi keratinocyte interleukin‐8 in human contact eczematous reactions to heavy metals
Author(s) -
Chen Libing,
Nordlind Klas,
Liden Sture,
Sticherling Michael
Publication year - 1996
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1996.tb04905.x
Subject(s) - staining , keratinocyte , chemistry , epidermis (zoology) , positive staining , cobalt chloride , chloride , pathology , biology , anatomy , medicine , biochemistry , cobalt , in vitro , inorganic chemistry , organic chemistry
Heavy metal‐induced contact eczematous human skin reactions to cobalt chloride and mercuric chloride were investigated for immunoreactivity to interleukin‐8 (IL‐8), by using an indirect immunoperox‐idase staining technique. There was suprabasal epidermal staining for IL‐8, with a decrease in the vicinity of areas with parakeratotic epithelium. However, in the immediate vicinity of a vesicular formation, intense staining of some apically situated keratinocytes was found. In addition, increased immunoreactivity over the acrosyringial area compared with the surrounding epidermis was obtained. These findings indicate an increased synthesis of keratinocyte IL‐8 in contact eczematous skin.