Premium
Generation of hydroxyl radicals by polymorphonuclear neutrophils during exposure to direct current and iron
Author(s) -
Fagerheim Ingrid,
Lingaas Egil
Publication year - 1996
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1996.tb04903.x
Subject(s) - radical , deoxyribose , chemistry , stimulation , oxygen , photochemistry , biophysics , biochemistry , medicine , organic chemistry , biology , dna
Human polymorphonuclear neutrophils were exposed to direct output current of 3 or 5 mA at either 300 or 500 V for 1 h in the presence and absence of iron. The current density was 25 or 40 nA/cm 2 . The formation of free oxygen radicals was measured as breakdown products from deoxyribose and methional. The cells were shown to generate oxygen radicals on electrical stimulation. A physiological concentration of iron enhanced radical formation, but OH was also formed in the absence of added iron. The most pronounced stimulation was seen at output current 5 mA and 500 V