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HTV‐1 proviral DNA sequences of env gp41 PCR amplificates from Tanzania
Author(s) -
HOLMHANSEN CAROL,
AYEHUNIE SEYOUM,
JOHANSSON BO,
NKYA WATOKY,
SHAO JOHN,
HAUKENES GUNNAR
Publication year - 1996
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1996.tb00742.x
Subject(s) - polymerase chain reaction , virology , biology , consensus sequence , gp41 , nucleic acid sequence , genome , dna , tanzania , dna sequencing , human immunodeficiency virus (hiv) , genetics , microbiology and biotechnology , gene , base sequence , antibody , epitope , environmental science , environmental planning
The polymerase chain reaction (PCR) and direct DNA sequencing were used to detect and characterize selected regions of the HIV‐1 proviral genome in whole blood samples from Tanzania. Specific PCR amplification products were obtained in gag and/or env (gp41) regions from 15 of the 19 HIV‐1 seropositive samples investigated. Env regions from 12 different amplificates were further characterized using the dideoxy sequencing method. Preliminary results indicate that, despite scattered nucleotide mismatches, HIV‐1 gp41 amino acid sequences from Tanzania conform to the 1990 Los Alamos African consensus sequence and resemble the HIV‐1 subtype A or D consensus sequences in the characterized regions.