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The interference of gingival cell cultures with growth of selected bacteria
Author(s) -
JOHANSSON ANDERS,
BERGENHOLTZ AXEL,
HOLM STIG E.
Publication year - 1996
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1996.tb00729.x
Subject(s) - fusobacterium nucleatum , microbiology and biotechnology , porphyromonas gingivalis , streptococcus mitis , actinobacillus , amnion , biology , staphylococcus epidermidis , hela , cell culture , bacteria , treponema denticola , staphylococcus aureus , streptococcus , genetics , pregnancy , fetus
The aim of the present study was to analyze the interference of oral tissue cells or cell lines ( effector cells ) with growth of reference bacteria, and furthermore to investigate whether cells derived from different individuals differ in such activity. The reference bacteria were Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus mitis, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis , and Fusobacterium nucleatum. The effector cells used were gingival fibroblasts (GF) from 21 periodontally involved persons, gingival epithelial cells (E) from 2 such persons, HeLa cells (HeLa), and an amnion cell line (Amnion). The cells were cultivated and their supernatants tested for antibacterial activity in a Bioscreen robot analyzer (Labsystems, Finland). Results suggest that the antibacterial activity of each tested primary cell line of tissue had its own profile depending on cell type and donor, and that the composition of oral microbiota was influenced by oral cells, which might, in turn, contribute to the variations in the pathogenesis of periodontal diseases.