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Determination of gamma/delta and other T‐lymphocyte subsets in bronchoalveolar lavage fluid and peripheral blood from patients with sarcoidosis and idiopathic fibrosis of the lung
Author(s) -
GRUBER R.,
PFORTE A.,
BEER B.,
RIETHMÜLLER G.
Publication year - 1996
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1996.tb00708.x
Subject(s) - bronchoalveolar lavage , sarcoidosis , medicine , cd8 , idiopathic pulmonary fibrosis , cd3 , immunology , lymphocyte , pathology , lung , t lymphocyte , immune system
We measured five different lymphocyte subpopulations, including γ/δ‐T cells, in peripheral blood and bronchoalveolar lavage (BAL) fluid from 48 consecutive patients undergoing diagnostic BAL (patients with sarcoidosis (n=19), patients with idiopathic pulmonary fibrosis (IPF; n=11) and patients with other diseases of the lung). Quantitative analysis of CD3+, CD4+, CD8+, CD16/56+ cells and γ/δ‐ T cells was done by flow cytometry. The proportion of CD3+ and CD4+ cells in the peripheral blood of patients with sarcoidosis and IPF was significantly diminished, while CD8+ lymphocytes and NK cells were significantly elevated compared to healthy controls. There was no significant difference for the γ/δ‐T cell subpopulation between patients with sarcoidosis, IPF and the control group. The proportion of CD3+ and CD4+ cells in BAL fluid was significantly elevated in sarcoidosis compared to IPF, while CD8+ cells were significantly diminished. Natural killer cells, defined as CD16/56+ CD3 – cells, showed comparable low numbers in sarcoidosis and IPF. For γ/δ‐T cells no significant difference was found between patients with sarcoidosis and IPF.