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Microbicidal mechanisms of liver macrophages in experimental visceral leishmaniasis
Author(s) -
KAUSALYA S.,
KAUR S.,
MALLA N.,
GANGULY N. K.,
MAHAJAN R. C.
Publication year - 1996
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1996.tb00704.x
Subject(s) - hydrogen peroxide , macrophage , lipopolysaccharide , kupffer cell , reactive oxygen species , peroxidase , leishmania donovani , leishmania , biology , microbiology and biotechnology , chemistry , visceral leishmaniasis , enzyme , immunology , biochemistry , leishmaniasis , parasite hosting , in vitro , computer science , world wide web
To study the differential microbicidal potentials of liver macrophages, the oxygen‐dependent and oxygen‐independent pathways in Kupffer cells and immigrant macrophages of Leishmania donovani ‐infected BALB/c mice were investigated. Hydrogen peroxide assay was performed using horse radish peroxidase‐dependent oxidation of phenol red to quantitate the reactive oxygen species produced. To examine the oxygen‐independent pathway, the enzymes N‐acetyl‐β‐glucosaminidase (NAG) and β‐glucuronidase (βG) were investigated after exposure of cells to lipopolysaccharide. Hydrogen peroxide release by Kupffer cells was significantly decreased only at 21 days postinfection, whereas hydrogen peroxide release by immigrant macrophages was significantly increased on all postinfection days with a maximum at 21 days postinfection. The pattern of release of NAG and βG was similar in both cell populations with a peak at 21 days postinfection. The present study therefore suggests that Kupffer cells and immigrant macrophages adopt different pathways to cope with this infection.