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Comparison of haemolytic activity between Fusobacterium necrophorum subsp. necrophorum and Fusobacterium necrophorum subsp. funduliforme in vitro and in vivo
Author(s) -
SHINJO TOSHIHARU,
MISAWA NAOAKI,
GOTO YOSHITAKA
Publication year - 1996
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1996.tb00689.x
Subject(s) - fusobacterium necrophorum , microbiology and biotechnology , hemolysin , in vivo , in vitro , haemolysis , biology , bacteroidaceae , fusobacterium , bacteria , virulence , immunology , biochemistry , gene , genetics , bacteroides
Haemolytic activity of two subspecies of Fusobacterium necrophorum was compared in vitro and in vivo. F. necrophorum subsp. necrophorum (Fnn) showed a stronger activity than F. necrophorum subsp. fiinduliforme (Fnf) in vitro. Haemolytic activity of Fnn and Fnf was 57.97%±1.90 and 17.33%±1.44, respectively, compared to complete haemolysis by distilled water. In the mice injected with Fnn, haemolysin was detected in the liver at a titre of from 1: 16 to 1: 128, and Fnn was recovered from all mice at a viable bacterial count of 10 5 to 10 6 cells per gram liver tissue. In the mice injected with Fnf, haemolysin titre was <1: 2 to 1: 32. No liver abscess was formed. The viable count of recovered bacteria was 10 3 to 10 5 cells per gram, except for two mice in which no Fnf was detected. The results suggest that haemolysin might be a virulence factor in this species.