Premium
Quantification of neutrophil chemotaxis: A comparison of stereological and 51 Cr‐labelling methods
Author(s) -
Storgaard M.,
West M. J.,
Nielsen S. L.,
Obel N.
Publication year - 1995
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1995.tb01094.x
Subject(s) - labelling , chemotaxis , incubation , stereology , fraction (chemistry) , variance (accounting) , chemistry , pathology , chromatography , medicine , biochemistry , receptor , accounting , business
A stereological method has been used to estimate the number of migrated polymorphonuclear leukocytes in a multipore filter assay. The estimates have been compared to those obtained with conventional 51 Cr‐labelling methods. The precision of the estimates in individual subjects with the two methods was similar, and in both cases the contribution to the interindividual variance made by the methods was small compared to the biological variance. However, the 51 Cr‐labelling method resulted in a significant reduction in the fraction of neutrophils that migrated as well as a delay in the chemo‐tactic response. Optimal assay conditions with regard to sample size, cell concentration, incubation time and chemoattractant fMLP concentration have been established for the stereological method and the virtues of the two techniques discussed.