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Inhibitory effect of lactoferrin on the adhesion of Actinobacillus actinomycetemcotnitans and Prevotella intermedia to fibroblasts and epithelial cells
Author(s) -
Alugupalli KISHORE R.,
Kalfas SOTIRIOS
Publication year - 1995
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1995.tb01091.x
Subject(s) - actinobacillus , prevotella intermedia , lactoferrin , bacteria , microbiology and biotechnology , pronase , adhesion , porphyromonas gingivalis , trypsin , biology , streptococcus mitis , chemistry , streptococcus , biochemistry , enzyme , genetics , organic chemistry
Adhesion of the periodontitis‐associated bacteria Actinobacillus actinomycetemcomituns and Prevotella intermedia to monolayers of fibroblasts, HEp‐2, KB and HeLa cells was quantified with radiolabeled bacteria. Bacterial adhesion was also examined microscopically with Giemsa‐stained nonradioactive preparations. The degree of bacterial adherence was dependent on the growth phase of the bacteria. Strains at the exponential phase adhered to a greater extent than those at the stationary phase of growth. Both human and bovine lactoferrins competitively inhibited the adhesion of A. actinomycetemcomitans and P. intermedia to all tested cell monolayers. The inhibitory effect was dose‐dependent in the concentration range 0.5–2500 μg/ml and not related to the bacterial growth phase. In the presence of lactoferrin, decreased association of bacteria with the cell monolayers was also found by microscopic examination of the preparations. The present findings indicate that lactoferrin may prevent the establishment of bacteria in periodontal tissues through adhesion‐counteracting mechanisms in addition to its bacteriostatic and bactericidal properties.