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Interferon‐γ and interleukin‐4 production by human T cells recognizing Leishmania donovani antigens separated by SDS‐PAGE
Author(s) -
Bahrenscheer JESPER,
Kemp MICHAEL,
Kurtzhals JØRGEN A. L.,
Gachihi GEORGE S.,
Kharazmi ARSALAN,
Theander THOR G.
Publication year - 1995
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1995.tb01088.x
Subject(s) - leishmania donovani , peripheral blood mononuclear cell , antigen , ionomycin , biology , visceral leishmaniasis , microbiology and biotechnology , interferon , interleukin 2 , immunology , cytokine , in vitro , biochemistry , leishmaniasis
Crude preparations of Leishmania donovani proteins were separated by preparative SDS‐polyacryl‐amide gel electrophoresis. Fractions of separated proteins were recovered by electroelution directly from the gel into separate chambers. The isolated protein fractions were tested for induction of proliferation and interferon‐γ (IFN‐γ) production in cultures of peripheral blood mononuclear cells (PBMC) from individuals who had recovered from visceral leishmaniasis caused by L. donovani. The release of interleukin‐4 (IL‐4) by PBMC stimulated with the isolated L. donovani antigen fractions was measured after treatment with phorbol‐myristate‐acetate and ionomycin. The cells proliferated in response to all protein fractions with molecular weights in the range <12 kDa to 85 kDa. In general, IFN‐γ was secreted in response to stimulation with all the protein fractions, whereas IL‐4 production was infrequently observed. The results show that T cells from individuals who have been cured of visceral leishmaniasis recognize and respond to a wide range of leishmanial antigens. There was no evidence of particular fractions constantly giving either IFN‐γ‐ or IL‐4‐producing responses.