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Ultrastructural localization of monoclonal antibodies against Pneumocystis carinii
Author(s) -
SUKURA ANTTI,
UKKOLA TARJA,
LINDER EWERT,
LINDBERG LARSAXEL
Publication year - 1994
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1994.tb05251.x
Subject(s) - pneumocystis carinii , monoclonal antibody , biology , ultrastructure , antibody , antigen , immunofluorescence , stain , staining , immunohistochemistry , protozoa , parasite hosting , microbiology and biotechnology , pathology , virology , immunology , human immunodeficiency virus (hiv) , anatomy , medicine , genetics , world wide web , computer science , pneumocystis jirovecii
The ultrastructural immunolocalization of target antigens recognized by two monoclonal antibodies against Pneumocystis carinii was investigated using both human‐ and rat‐derived organisms. Labelling was seen using both cryo electron microscopy and postembedding in Epon after fixation in glutaralde‐hyde. The antibodies showed different host species‐ and developmental stage specificity. One of the antibodies reacted with the outer membrane of all developmental stages of human‐derived but not rat‐derived organisms, whereas the other reacted only with the cyst form. The distribution of the latter was similar to that reported for methenamine silver, a widely used cytochemical stain for the parasite, and reacted with both human‐ and rat‐derived organisms. The antibodies described appear to be useful markers in studies on the differentiation of Pneumocystis carinii .