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Antigen‐specific suppression of antibody responses by T lymphocytes cytotoxic for antigen‐presenting cells
Author(s) -
Kagaya KEIKO,
Shang HONG,
Miyakawa YOZO,
Fukazawa YOSHIMURA
Publication year - 1994
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1994.tb04896.x
Subject(s) - cytotoxic t cell , antigen , microbiology and biotechnology , cd8 , splenocyte , biology , monoclonal antibody , cytotoxicity , antibody , immunology , chemistry , in vitro , biochemistry
In this study we demonstrated an alternative model of the antigen (Ag)‐specific suppression of antibody response in mice. Splenocytes that were taken from BALB/c mice immunized by i.v. injection of soluble human serum albumin (HSA) or ovalbumin exhibited MHC‐restricted Ag‐specific cytotoxicity for the respective antigen‐presenting cells (APC). When HSA‐primed splenocytes cultured with Ag and interleukin‐2 (IL‐2) were treated with anti‐CD4 or anti‐CD8 monoclonal antibody (mAb) plus complement, CD8 + and CD4 + T cells exhibited nearly the same level of cytotoxicity against APC. Furthermore, HSA‐primed CD4 + and CD8 + T cells released the same amount of interferon‐γ (IFN‐γ) when stimulated with Ag and IL‐2. Recombinant IFN‐γ was shown to suppress the in vitro plaque‐forming cell (PFC) response to sheep red blood cells (SRBC) only when it was added within 24 h after addition of Ag. The supernatants from both HSA‐primed CD4 + and CD8 + T cells suppressed the PFC response to SRBC in vitro , and the suppressive activity was abrogated by anti‐IFN‐γ mAb, but increased by anti‐IL 4 mAb. These results suggest that in our system the effector cells for Ag‐specific suppression of the antibody response in mice are both the cytotoxic type 1 clones (IFN‐γ‐producing) of CD4 + and CD8 + T cells for APC, and that IFN‐γ is a major extracellular effector molecule for such suppression.