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Identification of coagulase‐negative staphylococci and typing of Staphylococcus epidermidis by a 4 h micromethod
Author(s) -
JarlØV JENS OTTO,
Nissen BENT,
Rosdahl VIBEKE THAMDRUP,
Espersen FRANK
Publication year - 1994
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1994.tb04875.x
Subject(s) - staphylococcus epidermidis , typing , staphylococcus haemolyticus , microbiology and biotechnology , staphylococcus , coagulase , antibiogram , biology , identification (biology) , staphylococcus aureus , antibiotics , bacteria , antibiotic resistance , genetics , botany
A new 4 h micromethod (Minibact‐S) for identification of coagulase‐negative staphylococci (CNS) important in human medicine, Staphylococcus epidermidis, Staphylococcus hominis. Staphylococcus haemolyticus and Staphylococcus saprophytics , has been investigated. The reproducibility for species identification was 100% and the reproducibility for the single reactions was 95%. When compared to a modified Kloos & Schleifer conventional identification method, discrepant identification was obtained with the Minibact‐S in only 8 of 382 clinical and reference strains. The system has been used for typing and biotyping of 201 S. epidermidis strains, and gave a subdivision in 69 different types; 4 related types accounted for 91 (45%) of the isolates, whereas 44 types were represented by only 1 isolate. The discriminatory index was 0.82. In a comparison with other typing methods performed on 81 isolates from 15 patients Minibact‐S gave about the same number of types as antibiograms. but in 20% of the patients extra types were obtained with Minibact‐S alone compared to antibiogram alone and vice versa. The Minibact‐S is of some value in the routine clinical microbiology laboratory as a first screening method for identification of coagulase‐negative staphylococci and typing of S. epidermidis in combination with the antibiogram.