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Serological testing for campylobacteriosis with sera forwarded for Salmonella and Yersinia serology
Author(s) -
MÆLAND JOHAN A.,
BEVANGER LARS,
ENGE JOHANNE
Publication year - 1993
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1993.tb00159.x
Subject(s) - serology , campylobacter jejuni , antibody , yersinia , campylobacteriosis , salmonella , microbiology and biotechnology , antigen , yersinia enterocolitica , virology , medicine , biology , immunology , bacteria , genetics
Single serum specimens forwarded for Salmonella and Yersinia serology, from a total of 250 patients, were tested for anti‐ C. jejuni antibodies of the IgG class. Anti‐Salmonella and anti‐ Y. enterocolitica O3 antibodies were examined by a microagglutination test and anti‐ C. jejuni antibodies by ELISA against a C. jejuni ultrasonicate before (ELISA) and after neutralization of antibodies which cross‐reacted with Helicobacter pylori antigens (ELISA‐Abs). Blood donor sera ( n = 50) and sera ( n = 40) from patients with various infectious diseases served as controls. A positive test for anti‐Salmonella antibodies was recorded in 4/250 (1.6%) of the patients, for anti‐Yersinia antibodies in 7/250 (2.8%), and for anti‐ C. jejuni antibodies in 7/250 (2.8%) in the ELISA; in 25/250 (10%) in the ELISA‐Abs. No mixed infection was recorded by the serological testing. The ELISA‐Abs showed a diagnostic specificity of 97.7%. Our results support the inference that diagnostic serology for enteropathogenic bacteria should include serology for C. jejuni , preferably by tests which exclude participation by antibodies which cross‐react with H. pylori antigens.