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Microinjection of HEp‐2 cells with coxsackie B1 virus RNA enhances invasiveness of Shigella flexneri only after prestimulation with UV‐inactivated virus
Author(s) -
MODALSLI KRISTIN R.,
MIKALSEN SVEINOLE,
BUKHOLM GEIR,
DEGRÉ MIKLOS
Publication year - 1993
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1993.tb00153.x
Subject(s) - virus , shigella flexneri , rna , virology , biology , microbiology and biotechnology , shigella , transfection , cell culture , escherichia coli , gene , genetics , biochemistry
Coxsackie B1 virus induces increased susceptibility to invasion by Shigella flexneri when HEp‐2 cells are inoculated with the complete virus. When RNA from the same virus was microinjected into cells, virus RNA was synthesized and new virus particles were formed, but the transfected RNA had no effect on bacterial invasiveness. However, when the cells were prestimulated with UV‐inactivated virus, the microinjected RNA induced an additional enhancement of bacterial invasiveness. Microinjected whole virus particles did not replicate and did not induce any change in bacterial invasiveness. The results indicate that an initial event in virus multiplication is necessary to achieve an effect of transfected viral RNA on invasion of S. flexneri .