Premium
Adenoid tissue lymphocyte subpopulations — evaluation of a quantitative analysis with flow cytometry
Author(s) -
HEMLIN CLAES,
CARENFELT CHRISTER,
HALLDÉN GUNILLA,
HED JAN,
SCHEYNIUS ANNIKA
Publication year - 1993
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1993.tb00145.x
Subject(s) - adenoid , flow cytometry , etiology , otitis , pathology , immunology , hyperplasia , adenoidectomy , medicine , lymphocyte , biology , surgery , tonsillectomy
Secretory otitis media (SOM) is a common childhood disease without a completely clarified etiology. A chronic inflammatory condition in the nasopharynx, presumably caused by an increased bacterial load, is one factor of probable etiological importance. In the present study a flow cytometric method for analysis of adenoid lymphoid cell populations was developed to facilitate quantitative comparisons between children with SOM and children without ear disease. Adenoids removed from 18 children due to adenoid hyperplasia and obstructive symptoms were studied. Results of the flow cytometric analysis correlated well with the findings from immunohistological studies of five of the adenoids. PCA‐1 and CD25 were found to be good markers of increased cellular activity after non‐specific stimulation in cell culture. It is concluded that the flow cytometric method is suitable for further quantitative analysis of adenoid tissue.