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Improved identification of Staphylococcus aureus using a new agglutination test Results of an international study
Author(s) -
CROIZE J.,
GIALANELLA P.,
MONNET D.,
OKADA J.,
ORSI A.,
VOSS A.,
MERLIN S.
Publication year - 1993
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1993.tb00137.x
Subject(s) - staphylococcus aureus , agglutination (biology) , microbiology and biotechnology , serotype , latex fixation test , micrococcaceae , direct agglutination test , fibrinogen , antibody , biology , medicine , bacteria , immunology , serology , genetics , biochemistry
A new reagent for the identification of Staphylococcus aureus , SLIDEX STAPH‐KIT, operates on the principle of a latex and red blood cell combination agglutination: red blood cells are coated with fibrinogen for the detection of clumping factor, and latex particles are sensitized with anti‐ S. aureus serotype 18 monoclonal antibody for the detection of protein A and antigen 18. French strains belonging to serotype 18 are methicillin‐resistant. The performance of this reagent was compared with STAPHYSLIDE and STAPHAUREX in Europe (France, Germany, Italy), in the United States and in Japan using 548 methicillin‐resistant S. aureus strains, 392 methicillin‐sensitive S. aureus strains, and 441 non‐ aureus staphylococci. The specificity of the three reagents was equivalent (98.8% for SLIDEX STAPH‐KIT, 99,1% for STAPHYSLIDE, 98.1% for STAPHAUREX). SLIDEX STAPH‐KIT (97.3%) was more sensitive than STAPHYSLIDE (93.5%) and STAPHAUREX (89.7%) for all S. aureus strains due to a higher rate of identified methicillin‐resistant S. aureus strains.