Premium
Expression of proliferative cell nuclear antigen (PCNA) in urinary bladder carcinoma. Evaluation of antigen retrieval methods
Author(s) -
MALMSTRÖM PERUNO,
WESTER KENNETH,
VASKO JANOS,
BUSCH CHRISTER
Publication year - 1992
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1992.tb04030.x
Subject(s) - proliferating cell nuclear antigen , staining , antigen , pathology , malignancy , immunohistochemistry , monoclonal antibody , urinary bladder , carcinoma , antigen retrieval , antibody , biology , medicine , immunology
Proliferative cell nuclear antigen (PCNA) expression was analyzed in formalin‐fixed and paraffin‐embedded specimens from patients with urinary bladder cancer using three different anti‐PCNA monoclonal antibodies. In 20 recent cases a positive correlation was found between the extent and intensity of PCNA staining and grade of malignancy. In 95 specimens, three to six years old, extensive positive staining was detected in 15 and 23% of grade 2B and 3–4 tumors, respectively. No equivalent staining was found in the grade 1 and 2A tumors. In material more than six years old, a remarkably weak staining was observed regardless of grade. Similarly, in a test of archival material of tonsils a very weak immuno‐reactivity was found as compared with fresh material. However, antigen retrieval by microwave heating of the tissue sections was possible in the majority of all cases, and the difference in extent and intensity of the staining between low and high grade tumors remained.