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Increased expression of adhesion proteins (MAC‐1) and altered metabolic response in human leukocytes exposed to surfactant in vitro
Author(s) -
ZETTERBERG GÖRAN,
CURSTEDT TORE,
EKLUND ANDERS,
FORSLID JAN,
HED JAN
Publication year - 1992
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1992.tb03986.x
Subject(s) - pulmonary surfactant , receptor , in vitro , receptor expression , stimulation , chemistry , endocrinology , phorbol , cell surface receptor , medicine , flow cytometry , monocyte , biology , immunology , biochemistry , signal transduction , protein kinase c
In this study the modulatory effects of a well‐defined surfactant preparation on blood leukocytes were investigated. The expression of the cell surface receptor MAC‐1 was analyzed by flow cytofluorometry, and the metabolic response was measured by a chemiluminescence technique. An increase (p<0.05) in the MAC‐1 receptor expression was observed in the granulocytes but not in the monocytes. There was a decrease in the metabolic response of the leukocytes after stimulation with phorbol myristate acetate (PMA) and a delay (p<0.01 for both) in the peak activity. Formyl‐methionyl‐leucyl‐phenylalanine (fMLP) caused an increased peak (p<0.01). Thus, the surfactant preparation had a modulatory effect on blood leukocytes with regard to the expression of the cell surface receptor MAC‐1 and the metabolic response.