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Tetracycline resistance genes in Kenyan hospital isolates of Salmonella typhimurium
Author(s) -
KARIUKI SAMUEL,
MIRZA NAZIR BEGUM,
WASTESON YNGVILD,
SENERWA DANIEL,
GATHUMA JOSEPH M.,
OLSVIK ØRJAN
Publication year - 1992
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1992.tb03977.x
Subject(s) - tetracycline , tetr , plasmid , microbiology and biotechnology , salmonella , biology , enterobacteriaceae , bacteria , gene , antibiotics , escherichia coli , genetics , gene expression , repressor
All 97 strains of Salmonella typhimurium isolated from patients at a hospital in Nairobi, Kenya, during 1988‐90 were resistant to tetracycline. The minimum inhibitory concentration (MIC) showed a large distribution range from 1 μg/ml to 128 μg/ml. The strains were heterogeneous with respect to plasmid content, but initially all strains possessed, in addition to other plasmids, a large 60‐, 63‐or 65‐MDa plasmid. The tetracycline resistance genes were characterized using oligonucleotide probes, and 20% of the resistant strains possessed tetracycline type A ( tet r A), 6% tet r B, and 4% tet r C genes. Three strains possessed both type A and B tetracycline resistance determinants, which were shown to be located on the large 65‐MDa plasmid. There was no correlation between strains isolated from stools, blood, cerebrospinal or epidural fluids, pus, or urine, with respect to the tetracycline genotypes, MIC values or plasmid content.