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Screening method for β‐lactamase substrate profiles
Author(s) -
SCHUMACHER H.,
NIR M.,
MANSA B.,
THOMSEN V. F.
Publication year - 1992
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1992.tb00900.x
Subject(s) - isoelectric focusing , reproducibility , substrate (aquarium) , diffusion , clinical microbiology , chromatography , isoelectric point , identification (biology) , chemistry , materials science , microbiology and biotechnology , biology , biochemistry , physics , enzyme , ecology , botany , thermodynamics
A disc diffusion method, based on the idea of Klundert , for screening of substrate profiles of β‐lactamases was developed in order to perform epidemiological studies. The method was tested against 30 different reference β‐lactamases and 59 clinical isolates known to produce TEM‐1, SHV‐1 and BRO‐1. The reproducibility and discriminating power of the disc diffusion method made it possible to differentiate between eight types of substrate profiles for the 30 reference β‐lactamases and to differentiate between TEM‐1, SHV‐1 and BRO‐1 from clinical isolates. In combination with analytical isoelectric focusing the disc diffusion method gives a reliable identification of β‐lactamases.