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Superoxide anion release from blood monocytes and alveolar macrophages in patients with diffuse lung fibrosis
Author(s) -
SHERSON D.,
NIELSEN H.,
FREDERIKSEN J.,
MILMAN N.,
STRUVECHRISTENSEN E.,
PETERSEN B. NUCHEL
Publication year - 1992
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1992.tb00891.x
Subject(s) - bronchoalveolar lavage , idiopathic pulmonary fibrosis , pneumoconiosis , medicine , sarcoidosis , hypersensitivity pneumonitis , pulmonary fibrosis , lung , pathology , fibrosis , superoxide , interstitial lung disease , pulmonary alveolus , immunology , respiratory disease , chemistry , biochemistry , enzyme
Superoxide anion release (O 2 − ) after stimulation with phorbol myristate acetate was measured in alveolar macrophages (AM) obtained by bronchoalveolar lavage and in blood monocytes from 47 patients with diffuse interstitial lung disease: idiopathic pulmonary fibrosis (N = 15), hypersensitivity pneumonitis (N = 7), pneumoconiosis (N = 6) and sarcoidosis (N= 19). Differential cell counts demonstrated a lymphocyte predominance in patients with hypersensitivity pneumonitis (HP) and sarcoidosis while the other groups had neutrophil predominance. No correlation between O 2 − activity in alveolar macrophages (AM) or blood monocytes (BM) compared to lung function (VC and diffusing capacity) could be demonstrated. Smoking pneumoconiotics had significantly decreased BM Of release (1.25 ± 0.25 (SEM) nmol/min/10 6 cells) and significantly increased AM/BM O 2 − ratios (2.04±0.26) compared to smokers with idiopathic pulmonary fibrosis (IPF) who had the following mean values: BM O 2 − release = 2.58±0.25 and AM/BM O 2 − ratio=0.86 ± 0.23. When matched for sex and smoking, a significantly increased AM/BM O 2 − ratio was seen among patients with HP (2.19 ± 0.98) in comparison with patients who had sarcoidosis (0.40±0.18). Patients with either HP or pneumoconiosis had generally elevated AM O 2 − release and reduced BM O 2 − release. These results suggest that environmentally related interstial lung disorders (HP and pneumoconiosis) may be associated with elevated AM O 2 − release relative to BM O 2 − release in comparison to non‐environmentally related disorders (IPF or sarcoidosis).