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Lipopolysaccharide is present in immune complexes isolated from sputum in patients with cystic fibrosis and chronic Pseudomonas aeruginosa lung infection
Author(s) -
KRONBORG GITTE,
SHAND GEOFFREY H.,
FOMSGAARD ANDERS,
HØIBY NIELS
Publication year - 1992
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1992.tb00858.x
Subject(s) - cystic fibrosis , sputum , pseudomonas aeruginosa , immune system , immunology , lipopolysaccharide , antigen , pathogenesis , antibody , lung , microbiology and biotechnology , immune complex , antigen antibody complex , respiratory disease , medicine , subclass , biology , pathology , bacteria , tuberculosis , genetics
Sputum samples from seven patients with cystic fibrosis and chronic P. aeruginosa lung infection were investigated for immune complexes by PEG precipitation and in two different complement binding assays. All seven patients were immune complex positive. The components involved in immune complex formation were identified by SDS‐PAGE and immunoblotting. We found P. aeruginosa lipopolysaccharide as a major antigen. Both core and O‐specific saccharide antigens could be demonstrated. IgG and IgA were the immunoglobulins involved, with IgG2 as the dominating IgG subclass. Lipopolysaccharide has a number of biological activities and its presence in sputum may have consequences for the pathogenesis of lung disease in cystic fibrosis.