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Murine monoclonal antibodies against pneumococcal capsular polysaccharide types 4,8,22F and 19A/19F
Author(s) -
KOLBERG JAN,
AABERGE INGEBORG S.,
JANTZEN ERIK,
LØVIK MARTINUS,
LERMARK GRO,
STEEN TORUNN
Publication year - 1992
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1992.tb00845.x
Subject(s) - monoclonal antibody , polysaccharide , microbiology and biotechnology , antibody , streptococcus pneumoniae , chemistry , biology , immunology , biochemistry , antibiotics
Monoclonal antibodies (MAbs) were produced against pneumococcal capsular polysaccharides after subcutaneous immunization of BALB/c mice with a 23‐valent vaccine (Pneumovax® N, Merck, Sharp & Dohme). Selected antibodies were tested in ELISA against individual polysaccharides from 23 different pneumococcal types and in a dot blot assay with heat‐killed whole bacteria adhered to nitrocellulose paper. Three MAbs (isotype IgM) were found to be specific for types 4, 8 and 22F, respectively, whereas one (isotype IgA) reacted both with 19A and 19F. Very mild acid hydrolysis of the capsular polysaccharides resulted in loss of reaction with the antibodies.