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Multivariate analyses of cellular fatty acids and carbohydrates of 1:2:1 and 2:4:2 spirochetes
Author(s) -
BRONDZ ILIA,
FIEHN NILSERIK,
OLSEN INGAR,
SJÖSTRÖM MICHAEL
Publication year - 1991
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1991.tb05192.x
Subject(s) - rhamnose , treponema denticola , fatty acid , biology , carbohydrate , chemotaxonomy , multivariate statistics , treponema , chemistry , biochemistry , microbiology and biotechnology , bacteria , polysaccharide , botany , taxonomy (biology) , mathematics , statistics , genetics , porphyromonas gingivalis , syphilis , human immunodeficiency virus (hiv) , immunology
Delimitation of small‐sized spirochetes of the oral cavity can be difficult. The endoflagella pattern has long served as the main criterion for taxonomic distinction. For approved species, e.g. Treponema denticola , several endoflagella patterns are observed, which indicates that this criterion is inadequate. The present study with GC and GC‐MS used fatty acids and carbohydrates of whole‐cell methanoly‐sates to distinguish 1:2:1 and 2:4:2 subgingival spirochetes. Thirteen fatty acids: C 12:0 , C 13:0 , C 14:0 , C iso‐14:0 , C 2‐OH‐14:0 , C 15:0 , C antc‐15:0 , C 16:0 , C iso‐16:0 , C 16:1 , C 18:0 , C 18:1 , and C 18:2 , and three carbohydrates: rhamnose, glucose, and glucosamine, were detected. The carbohydrate contents did not differ between the two groups. While 1:2:1 spirochetes contained C iso‐14:0 and C antc‐15:0 acid, 2:4:2 spirochetes did not. Also multivariate analyses of quantitative fatty acid data distinguished between these groups.