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Characterization of β‐lactam‐resistant Klebsiella oxytoca isolated in a neonatal intensive care unit
Author(s) -
JALAKASPÖRNULL K.,
DORNBUSCH K.,
KÜHN I.,
RANSJÖ U.,
JONSSON C.,
BROBERGER U.
Publication year - 1991
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1991.tb05186.x
Subject(s) - klebsiella oxytoca , ceftazidime , cefotaxime , aztreonam , microbiology and biotechnology , klebsiella , plasmid , piperacillin , ampicillin , biology , phenotype , beta lactamase , antibiotics , enterobacteriaceae , imipenem , bacteria , antibiotic resistance , genetics , gene , escherichia coli , pseudomonas aeruginosa
The occurrence of Klebsiella oxytoca resistant to ampicillin, piperacillin, aztreonam and cefuroxime in a neonatal intensive care unit, including two cases of septicemia, was shown to consist of a spread on three consecutive occasions caused by three different biochemical Klebsiella oxytoca phenotypes. All isolates, except six surface isolates from one infant belonging to phenotype 1, were sensitive to cefotaxime (MIC 0.5–4 mg/1) and ceftazidime (MIC 0.25–1 mg/1). Isolates of phenotypes 1 and 2 produced a β‐lactamase with an isoelectric point of 5.5 and isolates of phenotype 3, a β‐lactamase with an isoelectric point of 7.9. The β‐lactamases of all three phenotypes hydrolysed benzylpenicillin and more slowly cephalothin. All phenotype 1 isolates carried a 2.9 Md plasmid and most isolates also a 36 Md plasmid. All phenotype 2 isolates carried a 4.8 Md plasmid and one isolate also a 30 Md plasmid. The phenotype 3 isolates carried only one 85 Md plasmid.