Premium
Identification and localization of a soluble antigen, Ag2, of 136 kDa from Plasmodium falciparum in vitro cultures
Author(s) -
JAKOBSEN P. H.,
GRELLIER P.,
THEANDER T. G.,
BEHRENDT N.,
TORII M.,
AIKAWA M.,
SCHREVEL J.,
JEPSEN S.
Publication year - 1991
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1991.tb05133.x
Subject(s) - immunogold labelling , antigen , immunoprecipitation , plasmodium falciparum , in vitro , microbiology and biotechnology , affinity chromatography , antibody , biology , immunofluorescence , immunoelectrophoresis , chemistry , biochemistry , immunology , malaria , enzyme
The soluble antigens, antigen 2 (Ag2) and antigen 6 (Ag6), were copurified from supernatants of P. falciparum in vitro cultures by affinity chromatography and Fast Protein Liquid Chromatography. Rabbit antibodies to Ag2 were raised and characterized by crossed immunoelectrophoresis. Ag2 appeared as a duplet with molecular masses of 136 and 120 kDa when tested by immunoblotting. Immunoprecipitation experiments on Triton X‐100 extracted antigens from synchronized cultures showed that the antigen was synthesized in the schizont stage. Ag2 was located near the surface of schizonts in the parasitophorous vacuole and in clefts in the infected erythrocyte cytoplasma as shown by immunogold electron microscopy.