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Production of antibodies to the constant region of the mouse T‐cell‐receptor β‐chain
Author(s) -
SCHENKEL JOHANNES,
SCHÄFER HUBERT,
KOCH SUSANNE,
BURGER REINHARD
Publication year - 1991
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1991.tb05126.x
Subject(s) - microbiology and biotechnology , complementary dna , receptor , antibody , antiserum , biology , cell surface receptor , clone (java method) , 5 ht5a receptor , cell , gene , biochemistry , immunology
To generate antibodies to the constant region of the mouse T‐cell‐receptor β chain, the corresponding cDNA clone 86T5 was subcloned into the bacterial expression vector pEX2. The induced hybrid protein consisting of a 30 kDa T‐cell‐receptor segment and a β‐galactosidase carrier moiety was used for immunization. In Western blots using lysates of a T‐cell‐receptor‐positive T‐cell hybrid, the antisera obtained reacted with a 42 kDa protein under reducing and a 85 kDa protein under nonreducing conditions. The antisera did not react in binding assays with intact T lymphocytes, i.e. membrane‐associated T‐cell‐receptor protein.