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Should aerobic blood cultures be shaken intermittently or continuously?
Author(s) -
Prag J.,
Nir M.,
Jensen J.,
Arpi M.
Publication year - 1991
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1991.tb01303.x
Subject(s) - vacutainer , bottle , incubation , agar plate , chemistry , chromatography , microbiology and biotechnology , bacteria , biology , biochemistry , materials science , composite material , genetics
Stationary incubation of aerobic blood cultures was compared with intermittent shaking in aerobic Vacutainer R 2630 bottles with agar slants during the first 24 h, and was simultaneously compared with the continuously shaken aerobic Bactec R 6A bottles as a reference system. Intermittent shaking did not significantly increase or decrease the seven days yield of the Vacutainer bottles as compared with the continuously agitated Bactec R 6A bottles. When one of 604 paired Vacutainer bottles was agitated for eight h and the other incubated stationary, the speed of growth detection was significantly greater in the agitated bottle (p< 0.001), but significantly less than with continuously agitated Bactec 6A bottles (p< 0.001). Agitation for 14 h showed the same pattern of detection speed. These results suggest that it is desirable initially to agitate aerobic blood cultures either intermittently or continuously.