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Evaluation of monoclonal antibodies in serovar classification of group B streptococci (GBS)
Author(s) -
Naess AUGUSTA IRENE,
Bevanger LARS,
Iversen OLEJAN,
Maeland JOHAN A.
Publication year - 1991
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1991.tb01300.x
Subject(s) - serotype , epitope , antiserum , antigen , monoclonal antibody , microbiology and biotechnology , biology , antibody , group a , group b , alpha (finance) , virology , medicine , immunology , construct validity , nursing , patient satisfaction
We have produced murine MAbs against the c protein from GBS and selected one anti‐c α and one anti‐c β MAb for serovar classification of c‐protein‐producing strains. A total of 61 GBS isolates, classified by serotype‐specific rabbit antisera, were tested in an immunofluorescent assay and whole‐cell ELISA. The MAbs permitted classification of the isolates into 13 serovars. A total of 34 strains contained the cα antigen, seven strains the c β antigen, and 20 strains both the cα and the c β antigen. The distribution of GBS with regard to c‐protein serovars was identical to that recorded when rabbit anti‐cα and ‐c β sera were used. The results show that the MAbs are directed against common epitopes on cα and β , respectively. The MAbs will be useful in the serovar determination of clinical GBS isolates and in the study of the structure and immunobiology of c proteins.