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Detection of genital papillomavirus types by polymerase chain reaction using common primers
Author(s) -
Jenkins ANDREW,
Kristiansen BJØRNERIK,
Ask EIRIK,
Oskarsen BENTE,
Kristiansen EWY,
Lindqvist BJØRN,
Trope CLAES,
KjØRstad KJELL
Publication year - 1991
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1991.tb01244.x
Subject(s) - primer (cosmetics) , polymerase chain reaction , oligonucleotide , biology , microbiology and biotechnology , typing , virology , dna , human papillomavirus , open reading frame , genome , genetics , gene , chemistry , medicine , peptide sequence , organic chemistry
We describe the detection of eight genital human papillomavirus (HPV) types, including HPV16 and HPV18, by PCR amplification of a 323 base‐pair region of the genome within the LI open reading frame (ORF). The primer sequences are: TGYAAATATCCWGATTWTWT and GTATCWACMA‐CAGTAACAAA. The method will detect purified HPV16 DNA down to a concentration of as little as a single molecule in 100 μl. The method is also applicable to purified DNA and crude lysates from tumour biopsies. Typing of the PCR product can be achieved with specific oligonucleotide probes.