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Early response in septicemia in newborns and their mothers
Author(s) -
Gutteberg TORE JARL,
Dalaker KNUT,
ØSterud BJARNE,
Vorland LARS H.
Publication year - 1991
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1991.tb01233.x
Subject(s) - streptococcus agalactiae , stimulation , tumor necrosis factor alpha , escherichia coli , lactoferrin , cytokine , medicine , endocrinology , chemistry , streptococcus , andrology , biology , bacteria , biochemistry , genetics , gene
Using a whole blood in vitro model, we have investigated the effect of Escherichia coli (E. coli), Streptococcus agalactiae (group B streptococci, GBS) and tumor necrosis factor alpha (TNF) on the generation of lactoferrin (LF), interleukin‐1 beta (IL‐1) and tissue thromboplastin (TPL) in healthy newborns at term and their mothers. E. coli (at a final concentration of 10 7 /ml) significantly increased the release of LF in whole blood from newborns after 20 as well as 60 min stimulation, and in samples from their mothers after 60 min stimulation. A significant increase in the release of LF was observed in both newborns and their mothers after 20 and 60 min stimulation with TNF (at a final concentration of 1000 pg/ml). A combination of TNF/ E. coli or TNF/GBS never gave any significant additional stimulatory effect. After stimulation with E. coli or GBS (both at a final concentration of 10 7 /ml) for 60 min a significant increase in production of TNF and TPL was observed in newborns. In newborns a significant increase in production of TNF and TPL was observed also after 20 min stimulation with E. coli. TNF (at a final concentration of 1000 pg/ml) significantly increased the generation of TPL after 20 and 60 min stimulation in both groups. There was a tendency for a greater release of LF and generation of TNF and TPL in samples from newborns compared with their mothers, but the differences were not statistically significant. E. coli , GBS and TNF had no significant effect on the production of IL‐1.