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Collagen distribution in developing experimentally induced granulation tissue
Author(s) -
Pedersen L. RYDE,
HØRslevPetersen K.,
Garbarsch C.,
Lorenzen I.
Publication year - 1990
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1990.tb05044.x
Subject(s) - sirius red , granulation tissue , viscose , anatomy , chemistry , pathology , biology , staining , wound healing , medicine , polymer chemistry , immunology
Viscose cellulose sponges were implanted subcutaneously on the back of full‐grown Sprague‐Dawley rats. Seven, 14, 21, 28, 42, 60 and 90 days after implantation, groups of 12 animals were decapitated and the sponges were removed and processed for light microscopy. Five um sections were stained with Picro‐Sirius Red. Morphometry was performed on the zone of ingrowth and the collagen. The intersectional variation in the morphometrically determined collagen density within the sponges was below 20%. The hydroxypro‐line content was determined biochemically in 5 um sections of sponges implanted for 14,42,60 and 90 days. A positive correlation (rho = 0.79, p < 0.0001) was observed between the biochemically and morphometrically determined collagen contents. The morphometric determinations showed a steady increase in the granulation tissue ingrowth. At day 60 the ingrowth was complete. There was an increasing collagen density from days 7 and 14 through days 21 and 28, followed by a nearly steady state up to day 90 and a significantly higher collagen density peripherally than centrally in the day 42 sponges. The study has shown that morphometric collagen determination at light microscopical level using Sirius Red‐stained sections may add quantitative data describing the dynamic changes in collagen content and distribution within developing granulation tissue.