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The influence of properties encoded by the Yersinia virulence plasmid on adhesion of Yersinia enterocolitica to ileal brush border membrane vesicles
Author(s) -
RæRregaard ANDERS,
Espersen FRANK,
Baker NEIL
Publication year - 1990
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1990.tb05017.x
Subject(s) - yersinia enterocolitica , microbiology and biotechnology , adhesion , plasmid , biology , chemistry , enterobacteriaceae , virulence , bacteria , biochemistry , escherichia coli , dna , gene , genetics , organic chemistry
The influence of plasmid‐associated cell surface structures on the ability of Yersinia enterocolitica to bind to ileal brush border membrane vesicles (BBVs) was investigated. Rabbit or human BBVs were immobilized on polystyrene microtiter plates and adhesion of radiolabeled cells of Y. enterocolitica was determined. Strains of pathogenic serotypes carrying the Yersinia virulence plasmid (pYV + ), as well as their isogenic plasmid cured derivatives (pYV), adhered to immobilized BBVs, but adhesion of pYV + organisms was markedly greater than that of pYV plus; ones. Strains belonging to non‐pathogenic serotypes did not adhere significantly. The pYV + strain Ye0301P+ did not express specific adhesion to glycolipids, nor was adhesion to BBVs reduced in the presence of various monosaccharides. Proteolytic digestion of surface structures on strain Ye0301P+ markedly reduced adhesion. pYV + strains also demonstrated greater adhesion to a non‐biological surface (polystyrene) and showed a higher degree of hydrophobicity than pYV” organisms as evaluated in a two‐phase partitioning system. It is therefore likely that the plasmid‐associated adhesion of Y. enterocolitica is promoted by one or more outer membrane proteins, that confer hydrophobicity to the bacterial surface.