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Local immune reaction in syngeneic mice against tumorigenic and nontumorigenic BPV‐transformed mouse cell lines
Author(s) -
Laatikainen AINO,
Sarkkinen HANNU,
SyrjÄNen KARI,
MantyjÄRvi RAUNO
Publication year - 1990
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1990.tb05014.x
Subject(s) - cytotoxicity , cytotoxic t cell , immunogenicity , cell culture , immune system , transplantation , antigen , biology , immunology , cell , cancer research , in vitro , medicine , genetics
The role of cytotoxic T cells in immune response to Bovine Papillomavirus type 1 (BPVl)‐transformed mouse cell lines was assessed. The chromium release assay was used to follow the induction of cytotoxicity in local lymph nodes of syngeneic C57BL/6J (B6) mice after injection of BPV1‐transformed cell lines tumorigenic in nude mice but tumorigenic or nontumorigenic in B6 mice. The nontumorigenic cell line B6B31. C‐NuT.A induced cell line‐specific cytotoxicity with a maximal activity on day 7 after subcutaneous inoculation of one million B6B31.C‐NuT.A cells. After injection of nontumorigenic B6B31.J or tumorigenic B6B31 J‐NuT. A or B6B31. A cell lines, only low levels of nonspecific cytotoxicity were observed. These results suggest a role for cytotoxic T cells only in rejection of B6B31. C‐NuT. A cells. One reason for the poor immunogenicity could be the lack of transformation‐induced/virus‐specific antigens on most of the present BPV1‐transformed cell lines. However, in a transplantation rejection experiment, protection was induced in B6 mice against a challenge of the highly tumorigenic B6B31.A‐B6T. 1 line by repeated injections of BPV1 ‐transformed cells. These results suggest the expression of common transplantation rejection antigens on the three BPV 1‐transformed cell lines used in the immunization experiments.