Premium
Neutrophil chemotactic activity of peptidoglycan A comparison between Staphylococcus aureus and Staphylococcus epidermidis
Author(s) -
Riber ULLA,
Espersen FRANK,
Wilkinson BRIAN J.,
Kharazmi ARSALAN
Publication year - 1990
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1990.tb05010.x
Subject(s) - staphylococcus epidermidis , chemotaxis , peptidoglycan , microbiology and biotechnology , staphylococcus aureus , alternative complement pathway , complement system , immunology , staphylococcal infections , staphylococcus , biology , bacteria , chemistry , biochemistry , antibody , receptor , genetics
The ability of peptidoglycan from Staphylococcus epidermidis and Staphylococcus aureus to generate in human serum chemoattractant for peripheral blood neutrophils was studied. It was shown that PG from the two bacteria was able to induce chemotactic activity in normal human serum. Sonication of PG was required to generate this activity. Very little or no activity was generated in heat‐treated or C5‐deficient human serum by PG, indicating that PG treatment of serum resulted in generation of chemoattractants by activation of complement. Kinetics studies employing C2‐deficient or MgEGTA‐chelated serum revealed that S. epidermidis induced chemotactic activity by activating the alternative complement pathway. The alternative complement activation induced by S. epidermidis occurred rapidly and was completed after 15 min, whereas S. aureus activated the alternative pathway much more slowly, with activation reaching a maximum at 60 min. The rapid activation of the alternative complement pathway by S. epidermidis PG may partly explain why this bacterium does not normally cause infections in healthy individuals.