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Detection of Cytomegalovirus‐infected cells in autopsy material by in situ hybridization
Author(s) -
Andersen CLAUS B.
Publication year - 1990
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1990.tb01045.x
Subject(s) - in situ hybridization , cytomegalovirus , virology , autopsy , biology , in situ , pathology , virus , medicine , herpesviridae , chemistry , viral disease , genetics , gene , messenger rna , organic chemistry
A non‐radioactive in situ hybridization (ISH) method was elaborated to detect cytomegalovirus (CMV)‐infected cells in tissue specimens processed for diagnostic routine histopathology. A biotinylated CMV‐DNA probe was hybridized following a) four different enzymatic predigestions, b) progressively increasing denaturation periods, and then detected by c) streptavidin‐biotin, d) a monoclonal antibody against biotin using a three‐stage alkaline phosphatase anti‐alkaline phosphatase (APAAP)‐technique, and e) combining c + d. Autopsy specimens obtained from an infant with acquired CMV‐infection, six patients with AIDS, five patients clinically and serologically without CMV‐infection, and preoperative needle core biopsies from six renal allografts served as material. ISH was specific and more sensitive when compared to immunohistochemical (IMH) detection of CMV‐antigens by a monoclonal antibody. ISH was concluded to be a rapid, practical, and sensitive tool in daily diagnostic histopathology.