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Binding of bacteria to carbohydrates immobilized on beads to demonstrate the presence of cell‐associated hemagglutinins in Vibrio cholerae
Author(s) -
Sanchez J.,
Jonson G.
Publication year - 1990
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1990.tb01043.x
Subject(s) - fucose , mannose , agglutination (biology) , microbiology and biotechnology , vibrio cholerae , hemagglutination , bacterial adhesin , bacteria , hemagglutinin (influenza) , agarose , vibrio , biology , biochemistry , vibrionaceae , chemistry , antigen , escherichia coli , glycoprotein , immunology , genetics , gene
We describe a phase constrast microscopy method for direct observation of adhesion of classical and El Tor vibrios to agarose beads containing covalently attached L‐fucose or D‐mannose. Binding of the vibrios to L‐fucose beads was found to correlate with fucose‐sensitive agglutination of human O erythrocytes, while binding of bacteria to beads with D‐mannose was consistent with mannose‐sensitive agglutination of chicken erythrocytes. Furthermore, vibrios expressing both fucose and mannose‐sensitive hemagglutinins adhered equally to L‐fucose and D‐mannose‐containing beads. Because this procedure is neither subject to biological variations in different populations of erythrocytes nor affected by other factors known to interfere with hemagglutination tests, it offers a suitable, more robust and specific alternative to detect functional adhesins in Vibrio cholerae and other bacteria.