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The pathology of Shigella flexneri infection in Rhesus monkeys: An endoscopic and histopathological study of colonic lesions
Author(s) -
Katakura SHIGEHIRO,
Reinholt FINN P.,
KÄRnell ANDERS,
Huan PHAM THI,
Trach DANG DUC,
Lindberg ALF A.
Publication year - 1990
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1990.tb01038.x
Subject(s) - lamina propria , pathology , shigellosis , pathological , immunohistochemistry , medicine , shigella flexneri , histopathology , intestinal mucosa , shigella , epithelium , biology , bacteria , salmonella , biochemistry , escherichia coli , gene , genetics
Twenty‐two Rhesus monkeys were orally fed 1 times 10 11 live virulent Shigella flexneri of either serotypes lb, 2a, 4a or Y. On the basis of colonoscopic findings they were classified into: group A – normal endoscopic picture (10 monkeys), and group B – pathological endoscopic picture (12 monkeys). Pathological findings, distributed over the entire colon, were seen as either red patches (± erosions) or diffuse lesions, i.e. fragile red mucosa, mucosal bleeding and broad edemas. Histopathological examination of concomitant biopsies showed an acute inflammation restricted to the mucosa in 8/12 of group B as compared to 2/10 of group A. The Shigellae were most commonly demonstrated in the surface epithelium and more rarely in the deep layer of the lamina propria. Immunohistochemical staining, using monoclonal antibodies directed against Shigella flexneri O‐antigenic polysaccharide, showed a high correlation with histopathological findings. Clinically all 10 monkeys in group A remained healthy, whereas 7/12 (all displaying histopathological signs of acute inflammation) in group B developed dysenteric symptoms. Colonoscopy should be combined with histopathological and immunohistochemical examinations of biopsies to study the pathological events taking place in the colon tissue during the course of a Shigella infection and will be of great value to assess the protective efficacy of S. flexneri vaccine candidates.