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Epidermal tumour necrosis factor α and interleukin 6‐like activities in AIDS‐related Kaposi's sarcoma
Author(s) -
OXHOLM ANNEMETTE,
OXHOLM PETER,
PERMIN HENRIK,
BENDTZEN KLAUS
Publication year - 1989
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1989.tb00827.x
Subject(s) - stratum spinosum , antiserum , tumor necrosis factor alpha , biology , pathology , cytokine , interleukin , immunology , polyclonal antibodies , stratum granulosum , necrosis , epidermis (zoology) , microbiology and biotechnology , medicine , virology , antibody , stratum corneum , anatomy
Biopsies from 6 patients with AIDS and Kaposi's sarcoma (KS) in the tumour stage, and 6 healthy controls, were immunohistologically examined for the presence of tissue‐bound tumour necrosis factorα (TNFα) and interleukin 6 (IL‐6) in the skin. TNFα was demonstrated using specific polyclonal antiserum to human recombinant TNFα. IL‐6 was visualized indirectly using a polyclonal antiserum to partially purified human crude supernatants of activated human blood monocytes, followed by absorption with recombinant human IL‐6. The cytokines were found identically located in epidermal cell membranes in stratum granulosum and spinosum of the epidermis from unaffected skin in both AIDS patients and in controls. Biopsies from KS elements showed markedly increased epidermal staining for both TNF δ and IL‐6. It was not possible to detect TNFα or IL6 in the endothelial cells of the tumour. The observation of increased amounts of epidermal‐bound TNFα and IL‐6 in AIDS‐related KS elements suplements previous studies indicating that the skin plays an active immunoinflammatory role in patients with AIDS.