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Pseudomonas Aeruginosa extracellular toxicity in infant mice and the protective effect of antitoxin A
Author(s) -
ØGAARD ALAIN REMI,
BERDAL BJØRN PETER
Publication year - 1989
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1989.tb00819.x
Subject(s) - antitoxin , pseudomonas aeruginosa , toxicity , extracellular , microbiology and biotechnology , biology , chemistry , medicine , bacteria , toxin , biochemistry , genetics
The toxin A‐content of crude extracellular preparations from Pseudomonas aeruginosa has been measured by ELISA. Using infant mice as test animal, the toxicity of these preparations was evaluated. The LD 50 in infant mice was determined at 80 ng of purified toxin A in saline. With ten μl of rabbit antitoxin A serum given together with purified toxin, the LD 50 increased to 2,500 ng. Generally there was no correlation between the LD 50 of the extracellular preparations, their quantity of toxin A as measured by ELISA, and the protective effect of antitoxin A.