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Pyrimidine 5‘‐nucleosidase in Neisseria meningitidis
Author(s) -
JYSSUM SIDSEL
Publication year - 1989
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1989.tb00797.x
Subject(s) - uridine , pyrimidine , uracil , cytidine , nucleotide , guanosine , chemistry , biochemistry , ribose , substrate (aquarium) , nucleoside , stereochemistry , biology , enzyme , rna , dna , gene , ecology
A previously unknown pyrimidine 5′‐nucleosidase (pyrimidine 5′‐nucleotide phosphoribo hydrolase) specific for uridine 5′‐monophosphate (5′‐UMP) was present in crude extract from Neisseria meningitidis. Of seven strains, six were found to degrade the N‐glycosidic linkage of 5′‐UMP. One strain showed negligible activity. The substrate was quantitatively converted to uracil and ribose 5′‐phosphate (R5P). Cytidine 5′‐monophosphate (5′‐CMP) or the 2‐deoxy 5′‐monophosphates of the pyrimidine nucleosides (5′‐dUMP, 5′‐dCMP, 5′‐dTMP) were found not to be degraded. Neither was uridine 3′‐monophosphate (3′‐UMP) degraded by extracts from the three strains selected for this test.