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Histopathological and immunohistochemical analysis of lung tumors: Description of a convenient technique for use with fine needle biopsy
Author(s) -
NORDGREN HANS,
NILSSON STEN,
RUNN ANNCHARLOTTE,
PONTEN JAN,
BERGH JONAS
Publication year - 1989
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1989.tb00768.x
Subject(s) - immunohistochemistry , pathology , biopsy , lung , medicine , needle biopsy , radiology
In this paper we describe a convenient procedure for use with fine‐needle biopsy in the diagnosis of lung and medistinal tumors. Cells obtained from a fine‐needle aspiration are suspended in a formaldehyde solution and allowed to sediment onto a polycarbonate filter, using a filtering device specially designed for this purpose. The filter with the aspirated cells is then embedded in paraffin and thereafter sectioned and stained in a routine manner. The main advantages with this technique, instead of cytology, are: 1. the cells are kept mainly intact, mostly in undisrupted clusters, and this increases the possibility of obtaining a specific diagnosis, a prerequisite for the diversified clinical management of malignant tumors; 2. possibilities exist, when necessary, of further immunohistochemical characterization of tumor cells in order to obtain a specific diagnosis without thoractomy. This could be of major importance when the management of certain tumors and infiltrates (i.e. those preferably treated by radio‐ and chemotherapy) does not require surgical intervention.