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Detection of antibody responses in rabbits hyperimmunized with Campylobacter pylori
Author(s) -
HÖÖK JOHANNA,
BLOMBERG BJÖRN,
DANIELSSON DAN,
KOSUNEN TIMO U.
Publication year - 1989
Publication title -
apmis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.909
H-Index - 88
eISSN - 1600-0463
pISSN - 0903-4641
DOI - 10.1111/j.1699-0463.1989.tb00755.x
Subject(s) - antiserum , campylobacter , microbiology and biotechnology , antigen , strain (injury) , campylobacter jejuni , antibody , serology , biology , immunoassay , immune system , glycine , immune sera , bacteria , immunology , amino acid , biochemistry , genetics , anatomy
Acid glycine extracts from four Campylobacter pylori strains and one GCLO strain were used as antigens in enzyme immunoassay (EIA). Immune responses of rabbits immunized with C. pylori strains, the GCLO‐strain and other campylobacter strains were studied. All 14 rabbit antisera against C. pylori reacted with all four C. pylori extracts and there were extensive cross‐reactions between these extracts. Antisera against C. jejuni and C. coli strains did not react with a C. pylori extract but reacted with the GCLO antigen. Acid glycine extracts proved to be very satisfactory in C. pylori serology and the use of an extract prepared from only one strain instead of a combination of strains seems justified.